Hey. I'm doing analysis on coursework which is the concentration of rennin and how long does it take for milk to clot.
I was wondering why is it that when the concentration doubles the rate of reaction doesnt?
my results show that it doesnt but i must have an explanation!
Thank you for the help!
There may be a point at which the enzyme is in excess (i.e. there is enough enzyme to work with all the casein in the milk, so adding any more doesn't make any difference. Before that point, the reaction may slow down as most of the casein will be locked onto enzyme so the remaining enzyme molecules take a little longer to 'find' a free casein. Finally, even from the same carton, the concentration of the casein in the milk may not be exactly uniform in different samples. You are assuming (as you have to) that the same quantity of milk from the same carton will keep the casein level constant, and it will in general terms, but not in absolute terms.