Hi, I am at planning stage for investigating water potential of potato cells. Method is to use sucrose solutions of different strengths i.e. 0.1M, 0.2M etc but I dont actually know how to get the exact quantities accurately. Can I use teaspoons or pipette & count the drops? I am doing this as home study. Am I exposing myself as having a major bolt lose in my head?!!
You can't make molar solutions of sucrose using teaspoons! To make a 1M solution of sucrose you would need to dissolve 342.2 grams in 1 litre of water. You can then dilute this to make your other solutions. Because you don't want that much solution, I would suggest you make the 1M solution by dissolving 34.2g in 100 cm3. You will need to be as accurate as you can with the liquid measurement (you could do with a measuring cylinder as a measuring jug won't really be accurate enough) and you will need a fairly accurate kitchen scales to measure the sucrose.