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catalase and concentration

Asked by rutendo | Sep 26, 2008 | AS Level > Biology > Coursework
rutendo
rutendo asks:

Explain how you would use apparatus toinvestigate effect of achanging substrate concentration on activity of catalase

Apparatus:
-conical flask
-rubber bung
-hydrogen peroxide solution-5%
-delivery tube
-beaker
graduated cylinder
distlled water
solution of catalase

etutor answers:

I can only do this briefly in an email. You measure the catralase activity by measuring the output of oxygen when the hydrogen peroxide breaks down due to the enzyme. From the apparatus you have, it sounds like you will do this by counting bubbles of oxygen coming out of the delivery tube into a beaker of water. You would count the number of bubbles in a given time. It mioght be possible (and more accurate) to invert the measuring cylinder (filled with water) over the delivery tujbe in a beaker and actually measure the oxygne given off. The more oxygen per unit time the greater the reaction. You would repeat the experiment using a range of concentrations of hydrogen peroxide.

2 student responses

rutendo
rutendo

Type your response here!

responded Oct 7, 2008 4:04:31 PM BST
rutendo
rutendo

I mean thanx a span

responded Oct 7, 2008 4:05:01 PM BST
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rutendo
rutendo
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